Human Annexin A2 (ANXA2) ELISA Kit

SKU: orb776560

Description

The Human Annexin A2 (ANXA2) ELISA Kit uses a sandwich enzyme immunoassay to quantify Annexin A2 (ANXA2) in human serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.058 ng/mL.

Research Area

Cancer Biology, Musculoskeletal & Connective Tissue Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ANXA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ANXA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ANXA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ANXA2 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

ANX-A2 ELISA Kit, ANX2L4 ELISA Kit, CAL1H ELISA Kit, LIP2 ELISA Kit, LPC2 ELISA Kit, LPC2D ELISA Kit, P36 ELISA Kit, PAP-IV ELISA Kit, Protein I ELISA Kit, Chromobindin-8 ELISA Kit, Calpactin-1 heavy chain ELISA Kit, Lipocortin II ELISA Kit, Placental anticoagulant protein IV ELISA Kit

Quick Database Links

UniProt

P07355