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Human Angiotensin II (AngII) ELISA Kit

Catalog Number: orb775263

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb775263
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin II(AngII).
ReactivityHuman
Concentration2000 pg/mL
Tested applicationsELISA
Application notesstandard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AngII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AngII in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range31.25-2000 pg/mL
Sensitivity9 pg/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesAng-II ELISA Kit, Angiotensin-2 ELISA Kit
NoteFor research use only
Human Angiotensin II (AngII) ELISA Kit