Human Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit

• Catalog Number: orb1807124
• Category: Assays and Kits
• Description: Human Angiotensin Ⅱ (Ang-Ⅱ) ELISA Kit
• Target: AngII
• Reactivity: Human
• UniProt ID: P01019
• Application notes: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-Ⅱ. During the reaction, Human Ang-Ⅱ in the sample or standard competes with a fixed amount of Human Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.
• Assay Type: Competitive
• Assay Time: 1.5H
• Standard: 2000pg/mL
• Range: 31.25-2000pg/mL
• Sensitivity: 13.7 pg/mL
• Sample Types: serum, plasma, Tissue homogenate and Other biological samples
• Alternative names: Angiotensin-2
• Research Area: Metabolism Research, Stem Cell & Developmental Bio
• Note: For research use only