Human Angiogenic Factor with G Patch and FHA Domains 1 (AGGF1) ELISA Kit

• Catalog Number: orb780044
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiogenic Factor With G Patch And FHA Domains 1(AGGF1).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: Q8N302
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGGF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGGF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGGF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGGF1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.061 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids.
• Alternative names: GPATC7 ELISA Kit, GPATCH7 ELISA Kit, VG5Q ELISA Ki
• Research Area: Signal Transduction
• Note: For research use only