Human ALPP Protein, His Tag

SKU: orb1743334

Description

Recombinant Human ALPP Protein with C-terminal 6×His tag

Key Properties

• Source: E.coli
• Expression System: HEK293
• Target: ALPP
• Reactivity: Human, Mouse, Rat
• Tag: C-6×His Tag
• Molecular Weight: The protein has a predicted molecular mass of 53.5 kDa after removal of the signal peptide. The apparent molecular mass of ALPP-His is approximately 55-70 kDa due to glycosylation.
• Expression Region: ALPP(Ile23-Thr505) 6×His tag
• Purification: Purified from cell culture supernatant by affinity chromatography
• Purity: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
• Reconstitution: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALP;ALPI;IAP;PALP;PLAP;PLAP-1

Greater than 95% as determined by reducing SDS-PAGE.

Immobilized Human TrkB-His at 2 μg/mL (100 μL/well) can bind Human BDNF*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human BDNF* is 47.58 ng/ml.

Quick Database Links

Gene Symbol

ALPP

UniProt

P05187