Human ALPL Protein, His Tag (active enzyme, MALS verified)

SKU: orb1743041

Description

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.3 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Source: Human ALPL Protein, His Tag (orb1743041) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Ser 502 (Accession # P05186-1).
• Expression System: HEK293
• Target: ALPL
• Reactivity: Human
• Tag: C-10×His
• Molecular Weight: 55.3 kDa
• Expression Region: Leu 18 - Ser 502
• Purity: 90%
• Endotoxins: 0.1 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -70°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 20 mM Tris,150 mM NaCl,pH7.5
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

ALPL

UniProt

P05186

RefSeq:P05186