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Human Alpha-Hemoglobin Stabilizing Protein (aHSP) ELISA Kit
Catalog Number: orb2667909
| Catalog Number | orb2667909 |
|---|---|
| Category | Assays and Kits |
| Description | Human Alpha-Hemoglobin Stabilizing Protein (aHSP) ELISA Kit |
| UniProt ID | Q9NZD4 |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human aHSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aHSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human aHSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aHSP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 1.57-100 ng/mL |
| Sensitivity | 0.47 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | A-HSP; ERAF; EDRF; Erythroid Associated Factor; Er Read more... |
| Research Area | Cardiovascular Research, Infectious Disease & Viro Read more... |
| Note | For research use only |
| Expiration Date | Please enquire. |
