Human Alpha-2-Macroglobulin (a2M) ELISA Kit

SKU: orb2668115

Description

Human Alpha-2-Macroglobulin (a2M) ELISA Kit

Images & Validation

• Application Notes:
  standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a2M in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Sample Types: serum, plasma, urine and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 µg/mL
• Sensitivity: 0.48 µg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

Quick Database Links

UniProt

P01023