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| Catalog Number | orb774918 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Acid Glycoprotein(a1AGP). |
| Reactivity | Human |
| Concentration | 400 ng/mL |
| UniProt ID | P02763 |
| Tested applications | ELISA |
| Application notes | standard: 400 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a1AGP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a1AGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a1AGP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human a1AGP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 6.25-400 ng/mL |
| Sensitivity | 2.76 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Alternative names | alpha1-AGP,AGP1 ELISA Kit, a1-AGP ELISA Kit, ORM1 Read more... |
| Note | For research use only |
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