Human Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) ELISA Kit

SKU: orb777175

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldehyde Dehydrogenase 1 Family, Member A1(ALDH1A1).

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALDH1A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALDH1A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALDH1A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALDH1A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.59 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ALDH11 ELISA Kit, ALDH1 ELISA Kit, ALDC ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, Retinaldehyde Dehydrogenase 1 ELISA Kit, Aldehyde dehydrogenase, cytosolic ELISA Kit, Retinal dehydrogenase 1 ELISA Kit

Quick Database Links

UniProt

P00352