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Catalog Number | orb2668114 |
---|---|
Category | Assays and Kits |
Description | Human Albumin (ALB) ELISA Kit |
UniProt ID | P02768 |
Application notes | standard: 100 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALB in the samples is then determined by comparing the OD of the samples to the standard curve |
Assay Time | 2.5h |
Range | 1.57-100 µg/mL |
Sensitivity | 0.48 µg/mL |
Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Alternative names | Serum albumin |
Note | For research use only |
Human | |
3.9 ng/mL-250 ng/mL | |
0.97 ng/mL |
Human | |
0.078 μg/mL-5 μg/mL | |
0.019 μg/mL |