Human Albumin (ALB) ELISA Kit

• Catalog Number: orb2668114
• Category: Assays and Kits
• Description: Human Albumin (ALB) ELISA Kit
• UniProt ID: P02768
• Application notes: standard: 100 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ALB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALB in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 1.57-100 µg/mL
• Sensitivity: 0.48 µg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Serum albumin
• Research Area: Cancer Biology, Gastroenterology & Hepatology, Hem
• Note: For research use only