Human Alanine Aminotransferase (ALT) ELISA Kit

SKU: orb776325

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alanine Aminotransferase(ALT).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 U/L. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ALT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ALT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ALT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 U/L
• Sensitivity: 0.059 U/L
• Concentration: 10 U/L

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GPT ELISA Kit, ALAT ELISA Kit, SGPT ELISA Kit, AAT1 ELISA Kit, GPT1 ELISA Kit, Serum Glutamic Pyruvic Transaminase ELISA Kit, Alanine Transaminase ELISA Kit, Glutamate pyruvate transaminase 1 ELISA Kit, Glutamic--alanine transaminase 1 ELISA Kit

Quick Database Links

UniProt

P24298