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Description
Research Area
Signal Transduction
Images & Validation
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Item 1 of 3
| Tested Applications | SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human |
|---|---|
| Biological Origin | Human |
| Target | AKT3 |
| Conjugation | Unconjugated |
| Purity | Recombinant protein corresponds to amino acids 1 to 481 of mature human AKT3; Akt isoform 3. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. |
Storage & Handling
−| Storage | Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: None. Stabilizer: 10% (v/v) Glycerol; Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10% glycerol |
| Concentration | 1.1 |
| Expiration Date | 6 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−RAC, PKB, AKT, PKB gamma
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001193658.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
WB
Western Blot (IB, immunoblot)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis