Human AKT (Total) ELISA Kit

SKU: orb2308026

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AKT in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Protein Biochemistry

Images & Validation

• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AKT in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.11 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

AKT serine/threonine kinase 3; AKT serine/threonine kinase 2; AKT serine/threonine kinase 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; thymoma viral proto-oncogene 1; D930002M15Rik; PRKBB; PRKBA; PRKBG; Nmf35; AKT; AI851531; PKBG; RAC-PK-gamma; PKBB; RAC; PKB/A; PKB-ALPHA; STK-2; RAC-gamma; PKB; PKB/Akt; PK; PKBBETA; AW554154; RAC-ALPHA; Akt; LTR-akt; MPPH; Nmf350; RAC-BETA; Rac; PKB-GAMMA; PKBbe; Ak; 2410016A19Rik; HIHGHH; MPPH2; PKBbeta; Pkbg; PKBalpha