Human Adropin (AD) ELISA Kit

SKU: orb780287

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adropin(AD).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AD in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 14.2 pg/mL
• Concentration: 2000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ENHO ELISA Kit, UNQ470 ELISA Kit, Energy Homeostasis Associated Protein ELISA Kit

Quick Database Links

UniProt

Q6UWT2

References

J Lis-Kuberka, M Berghausen-Mazur Evaluation of Selected Pro-and Anti-Inflammatory Adipokines in Colostrum from Mothers with Gestational Diabetes Mellitus International Journal of Molecular Sciences, (2024)

PubMed:

Applications

ELISA

Reactivity

Human