Human Adrenomedullin 2 (ADM2) ELISA Kit

• Catalog Number: orb778874
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Adrenomedullin 2(ADM2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adrenomedullin 2(ADM2).
• Tested applications: ELISA
• Reactivity: Human
• Range: 312.5-20000 pg/mL
• Assay Time: 2.5h
• Concentration: 20000 pg/mL
• UniProt ID: Q7Z4H4
• Sensitivity: 91.1 pg/mL
• Alternative names: AM2 ELISA Kit, IMDL ELISA Kit, IMDS ELISA Kit, Int
• Note: For research use only
• Application notes: standard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ADM2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADM2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.