Human Adiponectin (ADP) ELISA Kit

SKU: orb775098

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin(ADP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 30 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ADP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ADP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ADP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.47-30 ng/mL
• Sensitivity: 75 pg/mL
• Concentration: 30 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GBP28 ELISA Kit, ApM1 ELISA Kit, AdipoQ ELISA Kit, Acrp30 ELISA Kit, ACDC ELISA Kit, APM1 ELISA Kit, C1Q And Collagen Domain Containing ELISA Kit, Adipocyte Complement-Related Protein Of 30 KDa ELISA Kit, Adipose Most Abundant Gene Transcript 1 ELISA Kit

Quick Database Links

UniProt

Q15848

References

K Pacia, M Kaźnica-Wiatr, M Hat Effect of alcohol abuse on selected markers of inflammation, hemostasis, and endothelial function K Pacia, M Kaźnica-Wiatr, M Hat, (2024)

PubMed:

Applications

ELISA

Reactivity

Human