Human Activin A Protein, His Tag

• Catalog Number: orb383533
• Category: Proteins
• Description: Human Latent Activin A, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ser 426 (Accession # AAH07858.1).
• Target: Activin A
• Conjugation: Unconjugated
• Tag: N-6×His
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: AAH07858.1
• UniProt ID: P08476
• MW: 45.9 kDa
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 45.9 kDa. The protein migrates as 15 kDa and 40-45 kDa under reducing (R) condition, and 24 kDa, 40-45 kDa and 60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 1.0 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human Latent Activin A, His Tag at 10 µg/mL (100 µL/well) can bind Human ACVR2A, Fc Tag with a linear range of 0.6-20 ng/mL (QC tested).
• Expression Region: Ser 21 - Ser 426
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Research Area: Immunology & Inflammation
• Note: For research use only
• NCBI: AAH07858.1
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human Activin A protein

Immobilized Human Latent Activin A, His Tag (Cat. No. orb383533) at 10 μg/mL (100 μL/well) can bind Human ACVR2A, Fc Tag (Cat. No. orb1101193) with a linear range of 0.6-20 ng/mL (QC tested).