Human Actinin Alpha 3 (ACTN3) ELISA Kit

SKU: orb1949888

Description

Human Actinin Alpha 3 (ACTN3) ELISA Kit

Research Area

Hematology, Infectious Disease & Virology

Images & Validation

• Application Notes:
  This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ACTN3. You can calculate the concentration of Human ACTN3 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenate
• Assay Type: Sandwich
• Assay Time: 3.5H
• Range: 0.32-20ng/mL
• Sensitivity: 0.19 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, MGC1172, MGC1175

Quick Database Links

Gene Symbol

ACTN3

UniProt

Q08043