Human 5-Hydroxytryptamine (5-HT) ELISA Kit

SKU: orb782318

Description

The Human 5-Hydroxytryptamine (5-HT) ELISA Kit is designed to quantify 5-Hydroxytryptamine (5-HT) in human serum, plasma. The kit has a detection range of 14.07-900 ng/mL and a sensitivity of 4.05 ng/mL.

Research Area

Gastroenterology & Hepatology, Infectious Disease & Virology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 5-HT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma
• Assay Type: Competitive
• Assay Time: 2h
• Range: 14.07-900 ng/mL
• Sensitivity: 4.05 ng/mL
• Concentration: 900 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

5HT ELISA Kit, Serotonin ELISA Kit, N-Methyl-Gamma ELISA Kit

Quick Database Links

Gene Symbol

5-HT