Human 28S ribosomal Protein S12, Mitochondrial (MRPS12) ELISA Kit

• Catalog Number: orb2307976
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 50 ng/mL
• UniProt ID: O15235
• Application notes: standard: 50 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MRPS12 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRPS12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MRPS12 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.28 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Alternative names: RPS12; MRPS12; MRPS34; PSMD7; HTR1B
• Note: For research use only