HSP70 Protein

SKU: orb1714302

Description

Human Recombinant HSP70 Protein

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ATPase Activity Assay, ELISA, FA, SDS-PAGE, WB
• Application Notes:
  This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.

Key Properties

• Source: Recombinant
• Expression System: E. coli
• Biological Origin: Human
• Biological Activity: ATPase active
• Target: HSP70 Protein
• Reactivity: Human
• Tag: His tag
• Purification: Affinity Purified
• MW: ~70 kDa
• Purity: >90%
• Protein Sequence: MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD

Storage & Handling

• Storage: -20°C
• Buffer/Preservatives: 50mM Tris/HCl, pH 7.5, 0.15M NaCl and 10% glycerol
• Concentration: Lot/batch specific. See included datasheet.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B

Quick Database Links

Gene Symbol

HSP70 Protein

UniProt

P0DMV9, P0DMV8