HSP27 antibody

• Catalog Number: orb344401
• Category: Antibodies
• Description: HSP27 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: G3.1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: This HSP27 monoclonal antibody was produced by repeated immunizations with a prokaryotic recombinant protein corresponding to the full length human hsp27 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:1,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with HSP27 in human tissues and cell lines. MCF-7 cells are recommended as a positive control. Cross reactivity with hsp27 from other mammalian sources is likely. No cross reactivity occurs with HSP70, HSP90 or HSP104.
• Conjugation: Unconjugated
• UniProt ID: P04792
• NCBI: 17647521
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-HSP27 antibody, mouse anti-Heat shock p
• Note: For research use only
• Application notes: This protein A purified monoclonal antibody against human Hsp27 has been tested for use in immunoblotting and ELISA and is suitable in immunoprecipitation, immunohistochemistry, and immunocytochemistry. The antibody recognizes a 27 kDa band corresponding to hsp27 in cell lysates from breast carcinoma. Both frozen sections and paraffin embedded material can be used for immunocytochemistry and immunohistochemistry.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HeLa whole cell lysate is loaded per lane using HSP27 antibody