HSC70 (HSP73) Antibody

SKU: orb1822478

Description

Mouse Anti-Human HSC70 (HSP73) Monoclonal IgG2a Kappa

Research Area

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: AM, ELISA, ICC, IF, IHC, IP, PBA, PLA, WB
• Dilution range: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-151 was sufficient for detection of HSC70 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a Kappa
• Clone No.: 1F2-H5
• Immunogen: Full length human HSC70
• Target: HSC70 (HSP73)
• Molecular Weight: 73kDa
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

HSPA8, HSC70, HSP73, Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, HSP71, HSC71, HSC54, HSC73, HSPA10, LAP1, NIP71

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western Blot analysis of Human Cell lysates showing detection of Hsc70 protein using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70 (Hsp73) recombinant protein.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.

Quick Database Links

Gene Symbol

HSC70 (HSP73)

UniProt

P11142

NCBI Gene ID

3312

RefSeq (Protein):NP_006588.1