HPV18 E7 Protein, His Tag

SKU: orb3158075

Description

Human papillomavirus (HPV) E6 and E7 viral oncoproteins play the pivotal role in driving the cells toward oncogenesis. In their process of replicating the viral genome, they can induce all the hallmarks of a cancer cell, i.e., uncontrolled cellular proliferation, angiogenesis, invasion, metastasis, and unrestricted telomerase activity along with the evasion of apoptosis and growth suppressors’ activity. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Also plays a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.0 kDa. The protein migrates as 14-17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: E. coli
• Target: E7
• Reactivity: Virus
• Tag: N-10×His
• Molecular Weight: 14.0 kDa
• Expression Region: Met 1 - Gln 105
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -70°C
• Form/Appearance: Liquid
• Buffer/Preservatives: 50 mM Tris, 500 mM NaCl, pH 7.5
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

E7

UniProt

P06788

RefSeq:P06788