You have no items in your shopping cart.
Page Not Found
Cart summary
| Catalog Number | orb782344 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Relaxin(RLN). |
| Tested applications | ELISA |
| Reactivity | Equine |
| Range | 31.25-2000 pg/mL |
| Assay Time | 3.5h |
| Concentration | 2000 pg/mL |
| Sensitivity | 9.28 pg/mL |
| Alternative names | RLXH1 ELISA Kit, Relaxin H1 ELISA Kit, Prorelaxin Read more... |
| Note | For research use only |
| Application notes | standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse RLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse RLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse RLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse RLN in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma and other biological fluids |
| Expiration Date | Please enquire. |
