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Horse Collagen Type III (COL3) ELISA Kit

Catalog Number: orb782050

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb782050
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Collagen Type III(COL3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Collagen Type III(COL3).
ReactivityEquine
Concentration1000 ng/mL
Tested applicationsELISA
Application notesstandard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL3 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time2.5h
Range15.63-1000 ng/mL
Sensitivity4.87 ng/mL
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesCollagen Type 3, Col 3, Collagen Type III, Col III
NoteFor research use only
Horse Collagen Type III (COL3) ELISA Kit