Horse Collagen Type I (COL1) ELISA Kit

SKU: orb782051

Description

Horse Collagen Type I (COL1) ELISA Kit

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Reactivity: Equine
• Sample Types: serum, plasma, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Competitive
• Assay Time: 2h
• Range: 23.44-1500 ng/mL
• Sensitivity: 7.29 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

COL1, COLI

Standard curve for orb782051 ELISA kit.

Quick Database Links

Gene Symbol

COL1