HIV-1 (HXB2) GP41 Protein, His Tag

SKU: orb1710800

Description

Infection by HIV-1 involves the fusion of viral and cellular membranes with subsequent transfer of viral genetic material into the cell. The HIV-1 envelope glycoprotein that mediates fusion consists of the surface subunit gp120 and the transmembrane subunit gp41. gp120 directs virion attachment to the cell-surface receptors, and gp41 then promotes viral-cell membrane fusion. A soluble, alpha-helical, trimeric complex within gp41 composed of N-terminal and C-terminal extraviral segments has been proposed to represent the core of th e fusion-active conformation of the HIV-1 envelope. Three N-terminal helices within the bundle form a central, parallel, trimeric coiled coil, whereas three C-terminal helices pack in the reverse direction into three hydrophobic grooves on the surface of the N-terminal trimer. This thermostable subdomain displays the salient features of the core structure of the isolated gp41 subunit and thus provides a possible target for therapeutics designed selectively to block HIV-1 entry.

Research Area

Infectious Disease & Virology

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 44.3 kDa. The protein migrates as Band dispersion when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Source: HIV-1 (HXB2) GP41 Protein, His Tag (orb1710800) is expressed from E. coli cells. It contains AA Ala 533 - Leu 856 (Accession # P04578).
• Expression System: E. coli
• Target: Envelope glycoprotein gp41/gp41 (HIV)
• Reactivity: Virus
• Tag: C-10×His
• Expression Region: Ala 533 - Leu 856
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 44.3 kDa
• Purity: 85%

Storage & Handling

• Storage: -20℃
• Form/Appearance: Liquid
• Buffer/Preservatives: 50 mM HEPES,150 mM NaCl, 0.05% DDM, pH7.5
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Envelope glycoprotein gp41/gp41 (HIV)

UniProt

P04578

RefSeq:P04578