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Catalog Number | orb257520 |
---|---|
Category | Proteins |
Description | HIV-1 GP120 Protein, His Tag |
Reactivity | Virus |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 53.8 kDa |
Target | Envelope glycoprotein gp120 / gp120 (HIV) |
UniProt ID | G4XFJ5 |
Protein Sequence | Gene Num.: AX149771 |
Expression System | HEK293 |
Biological Origin | HIV-1 (CN54) |
Expression Region | Thr 36 - Arg 507 |
Endotoxins | 1.0 EU per μg |
NCBI | AX149771 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | GP120,GP120-CN54 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.8 kDa. The protein migrates as 80-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Rao, Vasudev R. et al. The gp120 protein is a second determinant of decreased neurovirulence of Indian HIV-1C isolates compared to southern African HIV-1C isolates PLoS One, 9, e107074 (2014)
SDS-PAGE analysis of Viral GP120 (HIV) protein
Unconjugated | |
95% | |
54.1 kDa | |
HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (orb257521) is expressed from human 293 cells (HEK293). It contains AA Thr 34 - Arg 506 (Accession # ABL67444.1). |
Unconjugated | |
95% | |
54.1 kDa | |
HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (orb257519) is expressed from human 293 cells (HEK293). It contains AA Thr 36 - Lys 511 (Accession # Q4QX31). |
98.00% | |
54.8 kDa (predicted); 95.49 kDa (reducing conditions) |
98.00% | |
55.8 kDa (predicted) |