HIST2H3C Antibody

SKU: orb344708

Description

Histone H3 K4me1 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ChIP, DOT, ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:3,000 - 1:10,000, ChIP: 2-5µg/million cells, IHC: 2 mg/ml - 5 µg/ml, IF: 2 - 5 µg/ml, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
• Target: HIST2H3C
• Purity: Anti-Histone H3 [Monomethyl Lys4] was purified from monospecific antiserum by protein A affinity purification. This antibody reacts with human Histone H3.2. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with many species including rat, chicken, Xenopus, Drosophila, and plant based on 100% sequence homology. Cross-reactivity with Histone H3 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782

Western Blot of Rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate (p/n orb348672). Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:5000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~15kDa for Histone H3.

Quick Database Links

Gene Symbol

HIST2H3C

UniProt

Q71DI3

RefSeq (Protein):NP_001005464.1