HIST1H3A Antibody

SKU: orb345948

Description

Histone H3 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
• Target: HIST1H3A
• Purity: Anti-Histone H3 is directed against the human histone 3 protein. The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic. Cross-reactivity with histone-3 from other sources have not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store H3 antibody at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.98 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Western Blot of Rabbit Anti-Histone H3 antibody. Lane 1: Hela WCL (p/n orb348668). Lane 2: Hela Nuclear Extract (p/n orb348671). Load: 10 µg per lane. Primary antibody: Histone H3 antibody at 1.0 µg/ml for 1 hr at RT. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: ~15 kDa, ~15 kDa for Histone H3.

Quick Database Links

Gene Symbol

HIST1H3A

UniProt

P68431

RefSeq (Protein):NP_003520.1