HIST1H3A Antibody

SKU: orb345947

Description

Histone H3 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 5.0 ug/ml, WB: 1.0 ug/ml
• Reactivity: Human
• Application Notes:
  Histone H3 antibody has been tested for use in ELISA and western blot. This antibody is suitable for IHC. For western blots expect a band of approximately 15.4 kDa in size corresponding to the Histone 3 protein. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Histone-3 was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the c-terminus region of human histone-3.
• Target: HIST1H3A
• Purity: Anti-Histone H3 is directed against the human histone 3 protein. The product was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. A BLAST analysis was used to suggest reactivity with human and multiple other eukaryotic. Cross-reactivity with histone-3 from other sources have not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store H3 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-histone H3 antibody, Histone H3.1, H3F, H3FA, H3FB, H3FL, H3FL, H3FC, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone 3 Antibody

Western Blot of Rabbit Anti-Histone H3 antibody. Lane 1: Hela WCL (p/n orb348668). Lane 2: Hela Nuclear Extract (p/n orb348671). Load: 10 µg per lane. Primary antibody: Histone H3 antibody at 1.0 µg/ml for 1 hr at RT. Secondary antibody: HRP Gt-a-Rb IgG secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Block: orb348637 overnight at 4°C. Predicted/Observed size: ~15 kDa, ~15 kDa for Histone H3.

Quick Database Links

Gene Symbol

HIST1H3A

UniProt

P68431

RefSeq (Protein):NP_003520.1