Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody

• Catalog Number: orb1152066
• Category: Antibodies
• Description: Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K20)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: H2BC3
• UniProt ID: P33778
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: H2BK20ac; Histone H2B(Acetyl Lys20); Acetyl Histon
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Cell type: HeLa + Serum starvation (12h) + SBA (5mM, 24h), Cross-linking conditions: No cross-linking, Amount of chromatin per IP: 5×106 cells, Amount of ab per IP: 4 ug, 12 ug, Beads type and amount per IP: 50 µl of Protein A/G MagBeads, Comment: The ChIP was performed with 1 µg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH Promoter, GAPDH CDS region, RPL30, FOXO3a-promoter and FOXO3a-downstream. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.