Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody

SKU: orb1152066

Description

Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H2B (Acetyl K20)
• Target: H2BC3
• Molecular Weight: 14 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2BC3 | Histone H2B (ac-K20); ac-Histone H2B; acetyl-Histone H2B; H2BK20Ac; ac-H2B; H2B.1; H2B/f; H2BFF; HIST1H2BB; H2B1B_HUMAN; H2BC3; H2B-clustered histone 3; Histone H2B.1; Histone H2B.f (H2B/f);

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: (-) HeLa, (+) HeLa + Sodium butyrate (30mM, 4hr), Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 14 kDa.

Cell type: HeLa + Serum starvation (12h) + SBA (5mM, 24h), Cross-linking conditions: No cross-linking, Amount of chromatin per IP: 5×106 cells, Amount of ab per IP: 4 ug, 12 ug, Beads type and amount per IP: 50 µl of Protein A/G MagBeads, Comment: The ChIP was performed with 1 µg of normal rabbit IgG as a negative control. Real time quantitative PCR was performed on immunoprecipitated DNA using primers specific for the human GAPDH Promoter, GAPDH CDS region, RPL30, FOXO3a-promoter and FOXO3a-downstream. Data are presented as enrichment of each sample relative to total amount of input chromatin at each amplicon.

Quick Database Links

Gene Symbol

H2BC3

UniProt

P33778