Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody

• Catalog Number: orb6166
• Category: Antibodies
• Description: Histone H2B (Acetyl K20) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Rabbit
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised acetylpeptide derived from human Histone H2B around the acetylation site of Lys20 IT(Ac-K)AQ
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 14 kDa
• Target: H2BC3
• UniProt ID: P33778
• RRID: AB_10943205
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Acetyl-Histone H2B(Lys20); Histone H2B(Acetyl-Lys2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Histone H2B (Acetyl K20) Polyclonal Antibody, Unconjugated (orb6166) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Histone H2B Polyclonal Antibody, Unconjugated (orb6166) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat testis tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Histone H2B (Acetyl K20) Polyclonal Antibody, Unconjugated (orb6166) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.