HIS Tag Antibody

SKU: orb2705217

Description

Goat polyclonal antibody to HIS epitope. The HHHHHH epitope is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transformed or transfected cells.

Images & Validation

• Tested Applications: WB
• Dilution range: WB:1:1,000-1:5,000
• Application Notes:
  The antibody solution should be gently mixed before use.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: EFPMHHHHKHHHHHHHHHHNPGRLERPHRD
• Purification: Epitope affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
• Concentration: 3 mg/ml
• Disclaimer: For research use only

Alternative Names

6xHis-Tag, HIS6 Tag, His Tag, HHHHHH Tag, antibody.