HighYield T7 mRNA Synthesis Kit (5moUTP)

SKU: orb1733512

Description

HighYield T7 ARCA mRNA Synthesis Kit (5moUTP) is designed to produce large amounts of 5-methoxyuridine-modified (m)RNA via in vitro transcription with T7 RNA polymerase. The resulting internally modified (m)RNA can subsequently be used for microinjection, transfection or in vitro translation experiments.\n5-Methoxyuridine modifications have been shown to increase (m)RNA stability and to reduce immunogenicity. A 5'-cap structure further increases translation efficiency. 5'-capping can be achieved by a) co-transcriptional capping with cap analoga (e.g. ARCA) or b) post-transcriptional capping with capping enzymes.\nThe kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) à 20 μl (7.5 mM GTP, 7.5 mM 5-Methoxy-UTP, 7.5 mM ATP, 7.5 mM CTP). An individual optimization of 5-Methoxy-UTP concentration can easily be achieved with the single nucleotide format.\nA 20 μl reaction yields about 100-130 μg RNA after 30 min incubation (1 μg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation)).

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Synthesis of 5-methoxyuridine-modified (m)RNA