HighFidelity IR680LT PCR Labeling Kit

SKU: orb1733326

Description

HighFidelity IR680LT PCR Labeling Kit is designed to produce ran-domly IR680LT-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. dUTP-XX-IR680LT is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpolymerase and a proofreading enzyme. 50% dUTP-XX-IR680LT substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of dUTP-XX-IR680LT/dTTP ra-tio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% dUTP-XX-IR680LT substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM dUTP-XX-IR680LT).

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λexc 679 nm, λem 694 nm, ε 190.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Storage & Handling

• Storage: store at -20 °C. avoid freeze/thaw cycles, store dark
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Preparation of IR680LT-labeled DNA probes by PCR

Compound Identifiers

PubChem CID

176418245