HighFidelity Fluorescein PCR Labeling Kit

• Catalog Number: orb653775
• Category: Tools
• Description: HighFidelity Fluorescein PCR Labeling Kit is designed to produce randomly Fluorescein-modified DNA probes by PCR. Such probes are ideally suited for Fluorescence in situ hybridization (FISH) and Northern Blot experiments. PCR-based labeling is superior to random-primed labeling with Klenow fragment if template amounts are limited or amplification of a specific DNA fragments is required. Amplification of probes up to 4 kbp is feasible. Fluorescein-12-dUTP is efficiently incorporated into DNA as substitute for its natural counterpart dTTP using an optimized reaction buffer and a High Fidelity Polymerase blend consisting of Taqpolymerase and a proofreading enzyme. 50% Fluorescein-12-dUTP substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of Fluorescein-12-dUTP/dTTP ra-tio however, can easily be achieved with the single nucleotide format. The kit contains sufficient reagents for 10 labeling reactions (S-Pack) or 50 labeling reactions (L-Pack) of 20 µl each (50% Fluorescein-12-dUTP substitution, 100 µM dATP/dGTP/dCTP, 50 µM dTTP, 50 µM Fluorescein-12-dUTP).
• SMILES: O=c1ccc2c(c1)oc1cc(O)ccc1c2c1c(ccc(c1)C(=O)NCCCCCC(=O)NC/C=C/c1cn(c(=O)[nH]c1=O)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)C(=O)O.O=c1c(cn(c(=O)[nH]1)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)/C=C/CNC(=O)CCCCCNC(=O)c1cc(c(c2c3ccc(O)cc3oc3cc(=O)ccc23)cc1)C(=O)O
• Storage: store at -20 °C
• Note: For research use only
• Application notes: < b>Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.