Hexanoyl-Lysine adduct Antibody (Biotin)

SKU: orb396561

Description

Mouse monoclonal antibody against Hexanoyl-Lysine adduct conjugated to Biotin.

Images & Validation

• Tested Applications: ELISA, FACS, FC, ICC, IF, WB
• Dilution range: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50)
• Reactivity: All
• Application Notes:
  A 1:1000 dilution of SMC-509 was sufficient for detection of Hexanoyl Lysine adduct in 0.5 µg of Hexanoyl Lysine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 500000000
• Immunogen: Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).
• Target: Hexanoyl-Lysine adduct
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Hexanoyl-Lysine adduct, HEL (Hexanoyl-Lysine adduct), HEL, HEL Adduct, Hexanoyl-Lys adduct, Hexanoyl-Lys, Hexanoyl-Lysine (HEL) adduct, Hexanoyl-Lys (HEL), Hexanoyl Lysine adduct, Hexanoyl-Lysine adduct-modified protein, Hexanoyl-Lysine Adduct (HEL)

Immunohistochemistry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Heart. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:26 for 2 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 489. Counterstain: DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Actin (C) Hexanoyl-Lysine adduct Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 μM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alexa Fluor 633 F-Actin stain. (E, F) Hexanoyl-Lysine adduct Antibody. (G, H) Composite.

Western Blot analysis of Hexanoyl Lysine-BSA Conjugate showing detection of 67 kDa Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Hexanoyl Lysine-BSA. Lane 3: BSA. Load: 0.5 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hexanoyl-Lysine adduct protein using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

Flow Cytometry analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG.

Quick Database Links

Gene Symbol

Hexanoyl-Lysine adduct