Hemin

SKU: orb1301729

Description

Hemin chloride is a chlorinated iron porphyrin and a potent inducer of heme oxygenase-1 (HO-1). It is used therapeutically in acute porphyrias to alleviate heme deficiency and suppress δ-aminolevulinic acid synthetase activity. This compound is widely applied in research models studying oxidative stress, inflammation, and cytoprotection in both cellular and animal studies.

Research Area

Cell Biology

Key Properties

• CAS Number: 16009-13-5
• MW: 651.94
• Purity: 99.59% (May vary between batches)
• Formula: C₃₄H₃₂ClFeN₄O₄
• SMILES: [Cl-].[Fe+3].Cc1c(CCC(O)=O)c2cc3[n-]c(cc4nc(cc5[n-]c(cc1n2)c(C=C)c5C)c(C=C)c4C)c(C)c3CCC(O)=O
• Target: Mitophagy|||Ferroptosis|||Autophagy
• Solubility: DMSO:30 mg/mL (46.02 mM);0.1M NaOH:25 mg/mL (38.35 mM);0.05%DMSO+99.95%PBS:0.33 mg/mL (0.51 mM);8% 0.1M NaOH+92% PBS:2 mg/mL (3.07 mM);0.05% DMSO+99.95% 0.1 M NaoH:0.33 mg/mL (0.51 mM)

Bioactivity

• Target IC50:
  SARS-CoV-2:0.6805 μM (EC50)|human caRD cellsiac voltage-gated sodium channel hNa V 1.5:80 nM|CYP1A2:27 μM|CYP3A4:2.4 μM|CYP2D6:19 μM|ANO1:0.45 μM
• In Vivo:
  METHODS: To investigate the effects on renal injury, Hemin (100 µmol/kg) was administered intraperitoneally to BABL/c mice with renal ischemia-reperfusion. RESULTS: Hemin pretreatment promoted ERK1/2 phosphorylation and enhanced tubular recovery, thereby preventing further kidney injury. METHODS: To investigate the effects on insulin resistance, Hemin (50 µmol/kg) was administered intraperitoneally to C57BL/6 mice on a high-fat diet once daily for four weeks. RESULTS: Hemin prevented the development of high-fat diet-induced insulin resistance by increasing insulin sensitivity in skeletal muscle.
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• In Vitro:
  METHODS: Pancreatic cancer cells PA-TU-8902, BxPC-3 and MiaPaCa-2 were treated with Hemin (30 µM) for 48 h. Cell viability was measured using the CellTiter-Glo Luminescent Cell Viability Assay. RESULTS: Hemin had a significant effect on cell proliferation of PA-TU-8902, BxPC-3 and MiaPaCa-2 cell lines, decreasing cell proliferation to 62±5%, 51±3% and 38±8%, respectively. METHODS: Astrocyte cultures were treated with Hemin (25 µM) for 12-24 h. Iron content was measured using colorimetric method. RESULTS: Iron accumulation occurred in cultured astrocytes after incubation with Hemin.
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• Cell Research:
  In vitro effects of various statins and hemin, a heme oxygenase inducer, on cell proliferation were evaluated in PA-TU-8902, MiaPaCa-2 and BxPC-3 human pancreatic cancer cell lines. The effect of statins on heme oxygenase activity was assessed and heme oxygenase-silenced cells were used for pancreatic cancer cell proliferation studies. Cell death rate and reactive oxygen species production were measured in PA-TU-8902 cells, followed by evaluation of the effect of cerivastatin on GFP-K-Ras trafficking and expression of markers of invasiveness, osteopontin (SPP1) and SOX2.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mitophagy, Mitochondrial Autophagy, Ferroptosis, Hemin, Hemin chloride, Autophagy, (HO)-1

Compound Identifiers

PubChem CID

156599052