Hematoxylin, PROBE

SKU: orb2652932

Description

This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure.

Images & Validation

• Application Notes:
  1. After chromogen staining, wash slide 2-3X with de/di water, 2X with 2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X. 3. Soak the slides in Immuno Automation buffer, PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue. 4. Wash slide 2-3X with de/di water. 5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount / limonene or equivalent after dehydrating the tissue with alcohols and organic solvents.

Storage & Handling

• Storage: Room Temperature
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only