Hematein

SKU: orb2901604

Description

Hematein is a oxidation product of hematoxylin acted as a dye. Hematein is an allosteric casein kinase II inhibitor with an IC50 of 0.74 μM. Hematein inhibits Akt/PKB Ser129 phosphorylation, the Wnt/TCF pathway and increases apoptosis in lung cancer cells.

Key Properties

• CAS Number: 475-25-2
• MW: 300.26
• Purity: >98% (HPLC)
• Formula: C₁₆H₁₂O₆
• SMILES: OC1=CC2=C3c4ccc(O)c(O)c4OCC3(O)CC2=CC1=O
• Target: Wnt/beta/catenin
• Solubility: In Vitro: DMSO : 100 mg/mL (333.04 mM; Ultrasonic )

Bioactivity

• In Vivo:
  Hematein (intraperitoneal injections; 50 mg/kg; twice a week for 6 weeks) inhibits tumor growth in A427 lung cancer cell xenografts. Animal model: Female athymic BALB/c nude mice (6-week-old). Dosage: 50 mg/kg. Administration: Intraperitoneal injections; twice a week for 6 weeks. Result: Inhibited tumor growth in A427 lung cancer cell xenografts.
• In Vitro:
  Hematein (10-100 μM; 14 days) inhibits cells growth in A427 lung cancer cells. Hematein (50 and 100 μM; 48 hours) induces apoptosis in A427 lung cancer cells. Hematein (50 and 100 μM; 48 hours) inhibits CK2-specific Akt phosphorylation. Cell Viability Assay Cell line: A427 (HTB-53) cell line. Concentration: 10-100 μM. Incubation time: 14 days. Result: Inhibited cells growth. Apoptosis Analysis Cell line: A427 (HTB-53) cell line. Concentration: 50 and 100 μM. Incubation time: 48 hours. Result: Induced apoptosis in A427 lung cancer cells. Western blot analysis. Cell line: A427 (HTB-53) cell line. Concentration: 50 and 100 μM. Incubation time: 48 hours. Result: Inhibited CK2-specific Akt phosphorylation.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Wnt/beta/catenin