Hamster Interleukin 10 (IL10) ELISA Kit

• Catalog Number: orb1146924
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10(IL10). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 10(IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10(IL10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10(IL10) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Hamster
• Range: 6.25-400 pg/mL
• Assay Time: 3.5h
• Concentration: 400 pg/mL
• Sensitivity: 2.64 pg/mL
• Alternative names: IL-10, CSIF, IL10A, TGIF, Cytokine Synthesis Inhib
• Note: For research use only
• Application notes: standard: 400 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster IL10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster IL10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster IL10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster IL10 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.