Hamster Cytochrome P450 7A1 (CYP7A1) ELISA Kit

• Catalog Number: orb1173591
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 7A1(CYP7A1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 7A1(CYP7A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 7A1(CYP7A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 7A1(CYP7A1) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Hamster
• Concentration: 20 ng/mL
• UniProt ID: P46634
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hamster CYP7A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hamster CYP7A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hamster CYP7A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hamster CYP7A1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.132 ng/mL
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Alternative names: CYP7, CYP7-A1, CYPVII, Cholesterol 7 Alpha Hydroxy
• Research Area: Metabolism Research
• Note: For research use only