HAMNO

SKU: orb1302962

Description

HAMNO (NSC-111847) is a small molecule inhibitor that selectively targets the replication protein A (RPA) complex. It is utilized in biochemical and cellular research to study DNA damage response pathways, replication stress, and cancer biology, with applications in both in vitro and in vivo experimental models.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 138736-73-9
• MW: 263.29
• Purity: 99.35% (May vary between batches)
• Formula: C₁₇H₁₃NO₂
• SMILES: Oc1ccccc1N\C=C1/C(=O)C=Cc2ccccc12
• Target: DNA/RNA Synthesis
• Solubility: DMSO:50 mg/mL (189.9 mM)

Bioactivity

• Target IC50:
  RPA:
• In Vivo:
  In mice, HAMNO inhibits the progression of UMSCC11B tumors. After treatment with etoposide (20 μM, 2 h), Ser33 of RPA32 (an ATR substrate) is highly phosphorylated, which is reduced with the addition of 2 μM HAMNO and is nearly absent at higher concentrations, demonstrating HAMNO can inhibit RPA32 phosphorylation by ATR.
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• In Vitro:
  HAMNO inhibits colony formation in both HNSCC cell lines. The combination of HAMNO with etoposide markedly inhibits colony formation to a greater degree than HAMNO alone. In UMSCC38 cells, HAMNO dose-dependently increased the occurs of pan-nuclear γ-H2AX staining. UMSCC38 and UMSCC11B cells have prominent γ-H2AX staining, particularly after incubation with HAMNO (20 μM). In UMSCC38 and OKF4 cells, HAMNO increased γ-H2AX staining, with the greatest increase in signal occurring in S-phase.
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• Cell Research:
  Cell cycle assessment and γ-H2AX staining are monitored in UMSCC38 and OKF4 cells after 2 h incubation with HAMNO (2, 20, 50 μM) and fixed in 70% ethanol overnight. Cells are washed with PBS and incubated overnight in PBS containing 1% BSA, 10% goat serum, and PS139-H2AX antibodies washed and incubated in goat anti-mouse Alexa Fluor 647 antibody for 30 min at room temperature. Cells are incubated in 50 μg/mL propidium iodide and 100 μg/mL RNase A for 30 min, and 10,000 cells per sample are analyzed.
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• Animal Research:
  UMSCC38 and UMSCC11B cells are implanted into 6-week-old female mice by a single subcutaneous injection of tumor cells (2 to 6×105 cells in 100 mL of sterile PBS). The growth rates of tumors are determined by daily monitoring of tumor volume with vernier calipers [tumor volume=1/2(length×width2)]. Once the tumor size reaches 50 mm3, etoposide (10 mg/kg mouse) and HAMNO (2 mg/kg) are administered intraperitoneally every day for 3 days. Tumor size is monitored daily and the volume of the tumor is compared among all experimental groups.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNASynthesis, DNA Synthesis, DNA replication, HAMNO, Inhibitor, inhibit, NSC111847, NSC-111847, NSC 111847, RNASynthesis, RNA Synthesis

Compound Identifiers

PubChem CID

85895