H2AFY Rabbit Polyclonal Antibody

• Catalog Number: orb583944
• Category: Antibodies
• Description: Rabbit polyclonal antibody to H2AFY
• Target: H2AFY
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Yeast, Zebrafish
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human H2AFY
• Protein Sequence: Synthetic peptide located within the following region: HPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARDNKKGRVTPRHILLAV
• UniProt ID: O75367
• MW: 40 kDa
• Tested applications: WB
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H2A.y, H2A/y, H2AFY, mH2A1, H2AF12M, MACROH2A1.1,
• Note: For research use only
• NCBI: NP_001035248

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Peptide is present in isoforms between 38 kDa and 40 kDa. Multiply modified by various post translational.

25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. MacroH2A.1 has many splice isoforms ranging from ~18 kDa to the canonical isoform at 40 kDa.

Sample Type: MCF7, Antibody dilution: 1.0 ug/ml. H2AFY is strongly supported by BioGPS gene expression data to be expressed in Human MCF7 cells.

WB Suggested Anti-H2AFY Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:12500, Positive Control: MCF7 cell lysate.