GW7647

SKU: orb1308239

Description

GW 7647 is a potent and selective PPARα agonist, demonstrating EC₅₀ values of 6 nM, 1100 nM, and 6200 nM for human PPARα, PPARγ, and PPARδ, respectively. Its ability to reduce nitric oxide in macrophages underpins its utility in studying lipid metabolism and inflammation in both cellular and animal models.

Research Area

Metabolism Research, Molecular Biology

Key Properties

• CAS Number: 265129-71-3
• MW: 502.75
• Purity: 99.89% (May vary between batches)
• Formula: C₂₉H₄₆N₂O₃S
• SMILES: CC(C)(Sc1ccc(CCN(CCCCC2CCCCC2)C(=O)NC2CCCCC2)cc1)C(O)=O
• Target: PPAR
• Solubility: DMSO:81.67 mg/mL (162.45 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (3.98 mM)

Bioactivity

• Target IC50:
  PPARα:6 nM (EC50)( Human PPARα)|PPARδ:6.2 μM (EC50)( Human PPARδ)|PPARγ:1.1 μM (EC50)( Human PPARγ)
• In Vivo:
  GW7647 (3 mg/kg per day) does not prevent cardiac hypertrophy but preserves left ventricular ejection fraction in vivo .
• In Vitro:
  GW7647 at a concentration of 50 nM, promotes PI3K and Akt (Ser473) phosphorylation, leading to an increase in NOS1 phosphorylation and subsequently elevating NO levels in stripped antral mucosa. Additionally, at the same concentration, it boosts the initial phase of Ca2+-mediated exocytotic events triggered by ACh in antral mucous cells, without independently inducing any exocytotic events. At a higher concentration of 1 μM, GW7647 substantially elevates PDZK1 protein levels to 129.7 ± 6.5% compared to controls in Caco2BBE cells, irrespective of IL-1β presence, and mitigates the IL-1β-induced reduction in PDZK1 expression. When combined with ACh, GW7647 augments the impact of wortmannin (50 nM) and AKT-inh (100 nM) on exocytotic activity in these cells. At 100 nM, GW7647 significantly decreases AQP9 protein levels by 43% in WIF-B9 hepatocytes and by 24% in HepG2 cells, though it shows no notable effects at 10 and 1,000 nM in WIF-B9 hepatocytes, nor does it significantly affect L-FABP protein levels in HepG2 hepatocytes.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GW 7647, GW7647, GW-7647, Inhibitor, inhibit, PPARδ, PPARγ, PPARα, PPAR, Peroxisome proliferator-activated receptors

Compound Identifiers

PubChem CID

3392731