Guinea pig Regulated on Activation in Normal T-Cell Expressed and Secreted (RANTES) ELISA Kit

SKU: orb779663

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted(RANTES).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig RANTES in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Guinea pig
• Sample Types: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCL5 ELISA Kit, SCYA5 ELISA Kit, SISd ELISA Kit, EoCP ELISA Kit, Chemokine C-C-Motif Ligand 5 ELISA Kit, Eosinophil Chemotactic Cytokine ELISA Kit, T-cell Specific Protein p288 ELISA Kit, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5 ELISA Kit