Guinea pig Nuclear Factor NF-kappa-B p105 subunit (NF-κB p105) ELISA Kit

SKU: orb782827

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B(NFkB).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Guinea pig
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.116 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

NF-KB1 ELISA Kit, EBP-1 ELISA Kit, KBF1 ELISA Kit, NF-Kappa-B ELISA Kit, NFKB-P105 ELISA Kit, NFKB-P50 ELISA Kit, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1 ELISA Kit, Nuclear factor NF-kappa-B p105 subunit ELISA Kit